TEAD4 antibody (C-Term)

Catalog No. ABIN6741569

This Rabbit Polyclonal antibody specifically detects TEAD4 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog, Pig and Bat.

Quick Overview for TEAD4 antibody (C-Term) (ABIN6741569)

Target: TEAD4 (TEA Domain Family Member 4 (TEAD4))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TEAD4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human TEAD4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human TEAD4 (Q96BK2, NP_003204). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Marmoset, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (92%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TEAD4

Target: TEAD4 (TEA Domain Family Member 4 (TEAD4))

Alternative Name: TEAD4

Background: Name/Gene ID: TEAD4

Synonyms: TEAD4, EFTR-2, HRTEF-1B, RTEF1, RTEF-1, TCF13L1, TEF-3, TEAD-4, TEF3, Transcription factor 13-like 1, Transcription factor RTEF-1, TEFR-1, TEA domain family member 4

Gene ID: 7004

NCBI Accession: NP_003204

UniProt: Q15561

Pathways: Regulation of Lipid Metabolism by PPARalpha