TULP2 antibody (N-Term)

Catalog No. ABIN6741571

Product Details anti-TULP2 Antibody

Target: TULP2 (Tubby Like Protein 2 (TULP2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TULP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TULP2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (92%) Rabbit (91%) Mouse, Rat, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human TULP2 (O00295, NP_003314). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Galago, Bat, Horse, Opossum (92%), Rabbit (91%), Mouse, Rat, Bovine (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TULP2

Target: TULP2 (Tubby Like Protein 2 (TULP2))

Alternative Name: TULP2 (TULP2 Products)

Synonyms: TULP2 antibody, CT65 antibody, TUBL2 antibody, Pdet antibody, tubby like protein 2 antibody, tubby-like protein 2 antibody, TULP2 antibody, Tulp2 antibody

Background: Name/Gene ID: TULP2

Synonyms: TULP2, Cancer/testis antigen 65, Tubby like protein 2, TUBL2, Tubby-related protein 2, Cancer testis antigen 65, CT65, Tubby-like protein 2

Gene ID: 7288

NCBI Accession: NP_003314

UniProt: O00295