ONECUT1 antibody (C-Term)

Catalog No. ABIN6741572

This Rabbit Polyclonal antibody specifically detects ONECUT1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Dog, Rabbit, Pig, Horse, Zebrafish (Danio rerio), Bat and Monkey.

Quick Overview for ONECUT1 antibody (C-Term) (ABIN6741572)

Target: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Reactivity: Human, Rat, Dog, Rabbit, Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ONECUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ONECUT1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human ONECUT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Pig (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ONECUT1 (Q9UBC0, NP_004489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Zebra finch (100%), Bovine (92%), Mouse, Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ONECUT1

Target: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Alternative Name: ONECUT1 / HNF6

Background: Name/Gene ID: ONECUT1

Synonyms: ONECUT1, Hepatocyte nuclear factor 6, HNF6, One cut domain family member 1, One cut homeobox 1, HNF-6, HNF6A

Gene ID: 3175

NCBI Accession: NP_004489

UniProt: Q9UBC0