HOXA5 antibody (C-Term)

Catalog No. ABIN6741580

The Rabbit Polyclonal anti-HOXA5 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect HOXA5 in samples from Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Drosophila melanogaster, Bat, Monkey, Chicken, Hamster and Xenopus laevis.

Quick Overview for HOXA5 antibody (C-Term) (ABIN6741580)

Target: HOXA5 (Homeobox A5 (HOXA5))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Drosophila melanogaster, Bat, Monkey, Chicken, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HOXA5 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human HOXA5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Pig, Guinea pig, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human HOXA5 (A2D5Y4, NP_061975). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Platypus, Lizard, Xenopus, Trout, Seabass, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish, Drosophila, Water flea, Beetle (100%), Smelt (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HOXA5

Target: HOXA5 (Homeobox A5 (HOXA5))

Alternative Name: HOXA5

Background: Name/Gene ID: HOXA5

Synonyms: HOXA5, Homeo box A5, Homeobox A5, Homeo box 1C, Homeobox protein Hox-1C, HOX1, Homeobox protein Hox-A5, Homeobox protein HOXA5, HOX1.3, HOX1C

Gene ID: 3202

NCBI Accession: NP_061975

UniProt: P20719