FUS antibody (N-Term)

Catalog No. ABIN6741586

The Rabbit Polyclonal anti-FUS antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FUS in samples from Human, Mouse, Rat and Monkey.

Quick Overview for FUS antibody (N-Term) (ABIN6741586)

Target: FUS (Fused in Sarcoma (FUS))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FUS Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human FUS

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Rat, Dog, Bovine, Rabbit, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human FUS. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Opossum, Platypus (100%), Dog, Bovine, Rabbit, Pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FUS

Target: FUS (Fused in Sarcoma (FUS))

Alternative Name: FUS / Gadd153

Background: Name/Gene ID: FUS

Synonyms: FUS, 75 kDa DNA-pairing protein, ALS6, CHOP, FUS-CHOP, FUS1, Fused in sarcoma, HnRNP-P2, HNRNPP2, Oncogene TLS, RNA-binding protein FUS, TLS, C/EBP-homologous protein, ETM4, Fus-like protein, Oncogene FUS, POMP75

Gene ID: 2521

UniProt: P35637