LIN7C antibody (N-Term)

Catalog No. ABIN6741597

The Rabbit Polyclonal anti-LIN7C antibody has been validated for WB, IHC and IHC (p). It is suitable to detect LIN7C in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey and Pig.

Quick Overview for LIN7C antibody (N-Term) (ABIN6741597)

Target: LIN7C (Lin-7 Homolog C (LIN7C))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN7C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIN7C Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human LIN7C

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken (100%) Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIN7C (Q9NUP9, NP_060832). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Salmon, Stickleback, Zebrafish (92%), Xenopus, Smelt (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LIN7C

Target: LIN7C (Lin-7 Homolog C (LIN7C))

Alternative Name: LIN7C / VELI3

Background: Name/Gene ID: LIN7C

Synonyms: LIN7C, LIN-7 protein 3, LIN-7-C, VELI3, Protein lin-7 homolog C, Vertebrate lin-7 homolog 3, Lin-7 homolog C (C. elegans), LIN-7C, MALS-3, MALS3, Mammalian lin-seven protein 3, Veli-3

Gene ID: 55327

NCBI Accession: NP_060832

UniProt: Q9NUP9

Pathways: Synaptic Membrane