ABCA5 antibody (AA 1296-1345)

Catalog No. ABIN6741601

The Rabbit Polyclonal anti-ABCA5 antibody (ABIN6741601) specifically detects ABCA5 in WB, IHC and IHC (p). The antibody is reactive with Human, Dog, Rabbit and Monkey samples.

Quick Overview for ABCA5 antibody (AA 1296-1345) (ABIN6741601)

Target: ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))

Reactivity: Human, Dog, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ABCA5 Antibody

Binding Specificity: AA 1296-1345

Specificity: Human ABCA5

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Dog, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1296-1345 of human ABCA5 (Q8WWZ7, NP_061142). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Mouse, Rat, Panda (92%), Bat (90%), Elephant, Dog, Rabbit, Horse, Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ABCA5

Target: ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))

Alternative Name: ABCA5

Background: Name/Gene ID: ABCA5
Subfamily: ATP-binding cassette - ABCA/ABC1
Family: Transporter

Synonyms: ABCA5, ATP-binding cassette A5, ABC13, EST90625, KIAA1888

Gene ID: 23461

NCBI Accession: NP_061142

UniProt: Q8WWZ7