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ABCA5 antibody (AA 1296-1345)

ABCA5 Reactivity: Human, Dog, Rabbit, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741601
  • Target See all ABCA5 Antibodies
    ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
    Binding Specificity
    • 5
    • 2
    • 1
    • 1
    • 1
    AA 1296-1345
    Reactivity
    • 18
    • 13
    • 12
    • 1
    • 1
    • 1
    • 1
    Human, Dog, Rabbit, Monkey
    Host
    • 18
    • 1
    Rabbit
    Clonality
    • 19
    Polyclonal
    Conjugate
    • 8
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ABCA5 antibody is un-conjugated
    Application
    • 7
    • 6
    • 5
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human ABCA5
    Predicted Reactivity
    Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Dog, Rabbit (85%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa1296-1345 of human ABCA5 (Q8WWZ7, NP_061142). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Mouse, Rat, Panda (92%), Bat (90%), Elephant, Dog, Rabbit, Horse, Opossum (85%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled Water.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
    Alternative Name
    ABCA5 (ABCA5 Products)
    Synonyms
    zgc:163009 antibody, ABC13 antibody, EST90625 antibody, B930033A02Rik antibody, mKIAA1888 antibody, ATP binding cassette subfamily A member 5 antibody, ATP-binding cassette, sub-family A (ABC1), member 5 antibody, ATP-binding cassette sub-family A member 5 antibody, ABCA5 antibody, abca5 antibody, LOC100545445 antibody, Abca5 antibody
    Background
    Name/Gene ID: ABCA5
    Subfamily: ATP-binding cassette - ABCA/ABC1
    Family: Transporter

    Synonyms: ABCA5, ATP-binding cassette A5, ABC13, EST90625, KIAA1888
    Gene ID
    23461
    NCBI Accession
    NP_061142
    UniProt
    Q8WWZ7
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