MED4 antibody (AA 146-195)

Catalog No. ABIN6741609

The Rabbit Polyclonal anti-MED4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect MED4 in samples from Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Chicken, Monkey, Xenopus laevis and Pig.

Quick Overview for MED4 antibody (AA 146-195) (ABIN6741609)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivity: Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Chicken, Monkey, Xenopus laevis, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED4 Antibody

Binding Specificity: AA 146-195

Brand: IHC-plus™

Specificity: Human MED4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human MED4 (Q9NPJ6, NP_054885). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Stickleback, Pike, Zebrafish (100%), Drosophila, Mosquito (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: MED4

Background: Name/Gene ID: MED4

Synonyms: MED4, ARC36, DRIP36, HSPC126, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36, TRAP36, VDRIP

Gene ID: 29079

NCBI Accession: NP_054885

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha