IMPDH1 antibody (AA 254-303)

Catalog No. ABIN6741618

This anti-IMPDH1 antibody is a Rabbit Polyclonal antibody detecting IMPDH1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey, Pig, Xenopus laevis, Chicken and Hamster.

Quick Overview for IMPDH1 antibody (AA 254-303) (ABIN6741618)

Target: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey, Pig, Xenopus laevis, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IMPDH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IMPDH1 Antibody

Binding Specificity: AA 254-303

Brand: IHC-plus™

Specificity: Human IMPDH1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Xenopus, Zebrafish (100%) Mouse, Dog, Rabbit, Horse, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human IMPDH1 (P20839, NP_899066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Zebrafish (100%), Rabbit, Stickleback (92%), Toxoplasma (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IMPDH1

Target: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Alternative Name: IMPDH1

Background: Name/Gene ID: IMPDH1

Synonyms: IMPDH1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPD1, LCA11, IMPD, IMPDH-I, SWSS2608, RP10

Gene ID: 3614

NCBI Accession: NP_899066

UniProt: P20839

Pathways: Ribonucleoside Biosynthetic Process