EGR2 antibody (AA 397-446)

Catalog No. ABIN6741623

The Rabbit Polyclonal anti-EGR2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect EGR2 in samples from Human, Mouse, Horse, Cow and Monkey.

Quick Overview for EGR2 antibody (AA 397-446) (ABIN6741623)

Target: EGR2 (Early Growth Response 2 (EGR2))

Reactivity: Human, Mouse, Horse, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EGR2 Antibody

Binding Specificity: AA 397-446

Brand: IHC-plus™

Specificity: Human EGR2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine (100%) Rat, Dog, Rabbit, Pig (92%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa397-446 of human EGR2 (P11161, NP_000390). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Bovine (100%), Rat, Hamster, Elephant, Panda, Dog, Rabbit, Pig, Guinea pig (92%), Galago, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EGR2

Target: EGR2 (Early Growth Response 2 (EGR2))

Alternative Name: EGR2

Background: Name/Gene ID: EGR2
Family: Transcription factor

Synonyms: EGR2, CMT1D, CMT4E, Early growth response 2, E3 SUMO-protein ligase EGR2, EGR-2, KROX20, Krox-20, AT591, Zinc finger protein Krox-20

Gene ID: 1959

NCBI Accession: NP_000390

UniProt: P11161