POLB antibody (AA 107-156)

Catalog No. ABIN6741696

This anti-POLB antibody is a Rabbit Polyclonal antibody detecting POLB in WB. Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Hamster, Bat and Monkey.

Quick Overview for POLB antibody (AA 107-156) (ABIN6741696)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Hamster, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLB Antibody

Binding Specificity: AA 107-156

Specificity: Human POLB / DNA Polymerase Beta

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Rabbit, Horse, Guinea pig (100%) Pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human POLB (P06746, NP_002681). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Elephant, Pig, Opossum (92%), Turkey, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB / DNA Polymerase Beta

Background: Name/Gene ID: POLB

Synonyms: POLB, DNA pol beta, Mutant DNA polymerase beta, DNA polymerase beta, DNA polymerase beta subunit

Gene ID: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: DNA Damage Repair