RAB5 antibody (AA 107-156)

Catalog No. ABIN6741707

This Rabbit Polyclonal antibody specifically detects RAB5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Monkey, Dog, Sheep, Horse, Rabbit, Pig, Hamster and Bat.

Quick Overview for RAB5 antibody (AA 107-156) (ABIN6741707)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Dog, Sheep, Horse, Rabbit, Pig, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAB5 Antibody

Binding Specificity: AA 107-156

Specificity: Human RAB5A / RAB5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Pig (100%) Guinea pig, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human RAB5A (P20339, NP_004153). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus (100%), Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Zebrafish (92%), Orangutan (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A / RAB5

Background: Name/Gene ID: RAB5A
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: RAB5A, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

Gene ID: 5868

NCBI Accession: NP_004153

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity