SHC3 antibody (AA 359-408)

Catalog No. ABIN6741713

The Rabbit Polyclonal anti-SHC3 antibody has been validated for WB. It is suitable to detect SHC3 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey and Pig.

Quick Overview for SHC3 antibody (AA 359-408) (ABIN6741713)

Target: SHC3 (SHC (Src Homology 2 Domain Containing) Transforming Protein 3 (SHC3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHC3 Antibody

Binding Specificity: AA 359-408

Specificity: Human SHC3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa359-408 of human SHC3 (Q92529, NP_058544). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Xenopus (90%), Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SHC3

Target: SHC3 (SHC (Src Homology 2 Domain Containing) Transforming Protein 3 (SHC3))

Alternative Name: SHC3 / SHCC

Background: Name/Gene ID: SHC3

Synonyms: SHC3, Neuronal Shc, NSHC, RAI, SH2 domain protein C3, SHC-transforming protein C, N-Shc, p52 and p64 isoforms of n-shc, Protein Rai, SHC-transforming protein 3, SHCC

Gene ID: 53358

NCBI Accession: NP_058544

UniProt: Q92529

Pathways: RTK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway