SHC3 antibody (AA 188-433)

Catalog No. ABIN7443536

The Rabbit Polyclonal anti-SHC3 antibody is suitable to detect SHC3 in samples from Human. It has been validated for WB and IHC.

Quick Overview for SHC3 antibody (AA 188-433) (ABIN7443536)

Target: SHC3 (SHC (Src Homology 2 Domain Containing) Transforming Protein 3 (SHC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SHC3 Antibody

Binding Specificity: AA 188-433

Purpose: Polyclonal Antibody to SHC-Transforming Protein 3 (SHC3)

Specificity: The antibody is a rabbit polyclonal antibody raised against SHC3. It has been selected for its ability to recognize SHC3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SHC-Transforming Protein 3 (SHC3) corresdonding to Val188~Gln433 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SHC3

Target: SHC3 (SHC (Src Homology 2 Domain Containing) Transforming Protein 3 (SHC3))

Alternative Name: SHC3

Background: N-Shc, NSHC, SHCC, Neuronal Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3

UniProt: Q92529

Pathways: RTK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway