P4HTM antibody (AA 323-372)

Catalog No. ABIN6741725

The Rabbit Polyclonal anti-P4HTM antibody has been validated for WB. It is suitable to detect P4HTM in samples from Human, Mouse, Rat, Horse, Cow, Dog, Rabbit, Guinea Pig, Pig, Bat and Monkey.

Quick Overview for P4HTM antibody (AA 323-372) (ABIN6741725)

Target: P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Rabbit, Guinea Pig, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HTM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-P4HTM Antibody

Binding Specificity: AA 323-372

Specificity: Human P4HTM

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human P4HTM (Q9NXG6, NP_808808). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Chicken, Platypus, Lizard, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for P4HTM

Target: P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))

Alternative Name: PH4 / P4HTM

Background: Name/Gene ID: P4HTM

Synonyms: P4HTM, EGLN4, HIF-PH4, HIF-prolyl hydroxylase 4, p4H with transmembrane domain, PH-4, p4H-TM, HIFPH4, HPH-4, p4HTM, PH4, PHD4, Proline 4-hydroxylase

Gene ID: 54681

NCBI Accession: NP_808808

UniProt: Q9NXG6