EGF antibody (AA 647-696)

Catalog No. ABIN6741736

This anti-EGF antibody is a Rabbit Polyclonal antibody detecting EGF in WB. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for EGF antibody (AA 647-696) (ABIN6741736)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGF Antibody

Binding Specificity: AA 647-696

Specificity: Human EGF

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse, Pig, Chicken (92%) Rat, Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa647-696 of human EGF (P01133, NP_001954). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Monkey, Marmoset, Panda, Bat, Horse, Pig, Turkey, Zebra finch, Chicken (92%), Xenopus (90%), Rat, Dog, Cat (85%), Lizard (84%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gene ID: 1950

NCBI Accession: NP_001954

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation