Fibronectin 1 antibody (AA 71-120)

Catalog No. ABIN6741737

This Rabbit Polyclonal antibody specifically detects Fibronectin 1 in WB. It exhibits reactivity toward Human, Cow, Pig, Guinea Pig, Horse, Monkey and Bat.

Quick Overview for Fibronectin 1 antibody (AA 71-120) (ABIN6741737)

Target: Fibronectin 1 (FN1)

Reactivity: Human, Cow, Pig, Guinea Pig, Horse, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Fibronectin 1 Antibody

Binding Specificity: AA 71-120

Specificity: Human FN1 / Fibronectin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Horse, Pig, Guinea pig (100%) Mouse, Rat, Chicken (92%) Xenopus, Zebrafish (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human FN1 (Q564H7, NP_473375). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Bovine, Bat, Horse, Pig, Opossum, Guinea pig (100%), Mouse, Rat, Chicken, Platypus, Lizard, Xenopus (92%), Zebrafish (91%), Medaka (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1 / Fibronectin

Background: Name/Gene ID: FN1

Synonyms: FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF

Gene ID: 2335

NCBI Accession: NP_473375

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy