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HPD antibody (AA 179-228)

The Rabbit Polyclonal anti-HPD antibody has been validated for WB. It is suitable to detect HPD in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey and Pig.
Catalog No. ABIN6741738

Quick Overview for HPD antibody (AA 179-228) (ABIN6741738)

Target

See all HPD Antibodies
HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Pig

Host

  • 72
  • 17
Rabbit

Clonality

  • 70
  • 19
Polyclonal

Conjugate

  • 43
  • 8
  • 8
  • 8
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
This HPD antibody is un-conjugated

Application

  • 55
  • 37
  • 30
  • 16
  • 14
  • 6
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 17
    • 9
    • 8
    • 8
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    AA 179-228

    Specificity

    Human HPD

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Horse, Guinea pig (100%) Pig (85%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa179-228 of human HPD (P32754, NP_002141). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Pig, Opossum, Xenopus, Beetle (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

    Alternative Name

    PPD / HPD

    Background

    Name/Gene ID: HPD

    Synonyms: HPD, 4HPPD, 4-HPPD, GLOD3, HPPDASE, PPD, Glyoxalase domain containing 3

    Gene ID

    3242

    NCBI Accession

    NP_002141

    UniProt

    P32754
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