RUNX1 antibody (C-Term)

Catalog No. ABIN6741816

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB. It exhibits reactivity toward Human, Chicken and Xenopus laevis.

Quick Overview for RUNX1 antibody (C-Term) (ABIN6741816)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX1 Antibody

Binding Specificity: C-Term

Specificity: Human RUNX1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chicken, Xenopus (100%) Dog, Bovine, Rabbit, Horse, Pig (92%) Mouse, Rat (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human RUNX1 (Q01196, NP_001001890). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Opossum, Turkey, Chicken, Lizard, Xenopus (100%), Monkey, Marmoset, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (92%), Mouse, Rat (91%), Gibbon, Zebra finch (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: AML1 / RUNX1

Background: Name/Gene ID: RUNX1

Synonyms: RUNX1, AMLCR1, Aml1 oncogene, AML1, AML1-EVI-1, CBF-alpha-2, CBFA2, Acute myeloid leukemia 1, EVI-1, PEBP2A2, PEA2-alpha B, AML1-EVI-1 fusion protein, Oncogene AML-1, PEBP2-alpha B, PEBP2aB

Gene ID: 861

NCBI Accession: NP_001001890

UniProt: Q01196