APIP antibody (AA 143-192)

Catalog No. ABIN6741857

The Rabbit Polyclonal anti-APIP antibody has been validated for WB. It is suitable to detect APIP in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Bat, Chicken, Hamster, Monkey and Pig.

Quick Overview for APIP antibody (AA 143-192) (ABIN6741857)

Target: APIP (APAF1 Interacting Protein (APIP))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Bat, Chicken, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APIP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APIP Antibody

Binding Specificity: AA 143-192

Specificity: Human APIP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Guinea pig, Chicken, Zebrafish (100%) Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human APIP (Q96GX9, NP_057041). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Catfish, Zebrafish (100%), Horse, Platypus, Xenopus, Frog, Salmon, Smelt, Stickleback, Pike, Mosquito (92%), Beetle (86%), Drosophila (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for APIP

Target: APIP (APAF1 Interacting Protein (APIP))

Alternative Name: APIP

Background: Name/Gene ID: APIP

Synonyms: APIP, APAF1-interacting protein, APIP2, CGI29, CGI-29, DJ179L10.2, MTRu-1-P dehydratase, APAF1 interacting protein, MMRP19

Gene ID: 51074

NCBI Accession: NP_057041

UniProt: Q96GX9

Pathways: Methionine Biosynthetic Process