EED antibody (AA 35-84)

Catalog No. ABIN6741861

This Rabbit Polyclonal antibody specifically detects EED in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Chicken, Monkey and Pig.

Quick Overview for EED antibody (AA 35-84) (ABIN6741861)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EED Antibody

Binding Specificity: AA 35-84

Specificity: Human EED

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig, Chicken (100%) Rabbit, Xenopus (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human EED (O75530, NP_003788). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (100%), Rabbit, Xenopus, Medaka (92%), Platypus (90%), Opossum, Stickleback (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:2500.

Not recommended for: IF, IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED

Background: Name/Gene ID: EED

Synonyms: EED, Embryonic ectoderm development, HEED, WAIT1, WAIT-1, Polycomb protein EED

Gene ID: 8726

NCBI Accession: NP_003788

UniProt: O75530