EGR1 antibody (AA 467-516)

Catalog No. ABIN6742004

This Rabbit Polyclonal antibody specifically detects EGR1 in WB. It exhibits reactivity toward Human, Horse, Cow, Dog, Sheep, Guinea Pig, Pig and Monkey.

Quick Overview for EGR1 antibody (AA 467-516) (ABIN6742004)

Target: EGR1 (Early Growth Response 1 (EGR1))

Reactivity: Human, Horse, Cow, Dog, Sheep, Guinea Pig, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGR1 Antibody

Binding Specificity: AA 467-516

Specificity: Human EGR1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Horse, Pig, Guinea pig (100%) Mouse, Rat (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa467-516 of human EGR1 (P18146, NP_001955). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Sheep, Elephant, Panda, Dog, Cat, Bovine, Horse, Pig, Guinea pig (100%), Mouse, Rat, Hamster (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: ZIF268 / EGR1

Background: Name/Gene ID: EGR1

Synonyms: EGR1, AT225, Early growth response 1, G0S30, KROX24, NGFIA, KROX-24, TIS8, ZIF-268, ZIF268, Zinc finger protein Krox-24, EGR-1, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225

Gene ID: 1958

NCBI Accession: NP_001955

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity