MYOD1 antibody (AA 251-300)

Catalog No. ABIN6742010

This Rabbit Polyclonal antibody specifically detects MYOD1 in WB. It exhibits reactivity toward Human.

Quick Overview for MYOD1 antibody (AA 251-300) (ABIN6742010)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MYOD1 Antibody

Binding Specificity: AA 251-300

Specificity: Human MYOD1 / MYOD

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human MYOD1 (P15172, NP_002469). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Marmoset (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD / MYOD1

Background: Name/Gene ID: MYOD1

Synonyms: MYOD1, BHLHc1, Myogenic differentiation 1, Myf-3, MYOD, Myogenic factor 3, PUM, MYF3

Gene ID: 4654

NCBI Accession: NP_002469

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development