Ephrin B1 antibody (AA 179-228)

Catalog No. ABIN6742068

The Rabbit Polyclonal anti-Ephrin B1 antibody has been validated for WB. It is suitable to detect Ephrin B1 in samples from Human, Mouse, Dog, Monkey, Guinea Pig and Rabbit.

Quick Overview for Ephrin B1 antibody (AA 179-228) (ABIN6742068)

Target: Ephrin B1 (EFNB1)

Reactivity: Human, Mouse, Dog, Monkey, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ephrin B1 Antibody

Binding Specificity: AA 179-228

Specificity: Human EFNB1 / Ephrin B1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit (100%) Rat, Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human EFNB1 (P98172, NP_004420). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Panda, Dog, Rabbit, Guinea pig (100%), Rat, Bovine, Bat (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: EFNB1 / Ephrin B1

Background: Name/Gene ID: EFNB1
Family: Ephrin

Synonyms: EFNB1, CFNS, EFL3, ELK ligand, Ephrin B1, Ephrin-B1, Elk-L, EFL-3, CFND, EPLG2, LERK-2, LERK2, Ligand of eph-related kinase 2

Gene ID: 1947

NCBI Accession: NP_004420

UniProt: P98172

Pathways: RTK Signaling