GAPDH antibody (AA 107-156)

Catalog No. ABIN6742093

This Rabbit Polyclonal antibody specifically detects GAPDH in WB. It exhibits reactivity toward Human.

Quick Overview for GAPDH antibody (AA 107-156) (ABIN6742093)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GAPDH Antibody

Binding Specificity: AA 107-156

Specificity: Human GAPDH

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human GAPDH (P04406, NP_002037). Percent identity by BLAST analysis: Human, Chimpanzee (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406