GAPDH antibody (AA 107-156)
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- Target See all GAPDH Antibodies
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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Binding Specificity
- AA 107-156
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Reactivity
- Human, Mouse, Rat, Rabbit, Pig, Dog, Monkey, Zebrafish (Danio rerio), Horse, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GAPDH antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Human GAPDH
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Zebrafish (100%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa107-156 of human GAPDH (P04406, NP_002037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Dog, Cat, Rabbit, Horse, Pig, Opossum, Guinea pig, Salmon, Zebrafish (100%), Hamster, Bat, Armadillo, Xenopus, Trout, Smelt, Pike (90%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product GAPDH Primary Antibody
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- Application Notes
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Alternative Name
- GAPDH (GAPDH Products)
- Background
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Name/Gene ID: GAPDH
Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit - Gene ID
- 2597
- NCBI Accession
- NP_002037
- UniProt
- P04406
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