GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (AA 107-156)

Catalog No. ABIN6742094

Product Details anti-GAPDH Antibody

Target: GAPDH

Binding Specificity: AA 107-156

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Monkey, Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human GAPDH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human GAPDH (P04406, NP_002037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Dog, Cat, Rabbit, Horse, Pig, Opossum, Guinea pig, Salmon, Zebrafish (100%), Hamster, Bat, Armadillo, Xenopus, Trout, Smelt, Pike (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH

Alternative Name: GAPDH (GAPDH Antibody Abstract)

Synonyms: G3PD, GAPD, Gapd, BEST:GH12586, CG12055, Dmel\\CG12055, GA3PDH, GADPH, GAP, GAPDH, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, g3pd, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase S homeolog, glyceraldehyde-3-phosphate dehydrogenase, type I, olfactory receptor 8K3, GAPDH, Gapdh, Gapdh1, gapdh, gapdh.S, gapDH, LOC100404960, LOC614985

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406