RPA1 antibody (AA 215-264)

Catalog No. ABIN6742105

This Rabbit Polyclonal antibody specifically detects RPA1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Sheep, Pig, Bat and Xenopus laevis.

Quick Overview for RPA1 antibody (AA 215-264) (ABIN6742105)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Sheep, Pig, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPA1 Antibody

Binding Specificity: AA 215-264

Specificity: Human RPA1 / RPA70

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human RPA1 (P27694, NP_002936). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Xenopus (100%), Marmoset, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Drosophila (92%), Zebrafish (91%), Salmon (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA70 / RPA1

Background: Name/Gene ID: RPA1

Synonyms: RPA1, HSSB, MST075, Replication factor A protein 1, Replication protein A1 (70kD), RF-A, RF-A protein 1, MSTP075, Replication protein A1, 70  kDa, RP-A, REPA1, RP-A p70, RPA70

Gene ID: 6117

NCBI Accession: NP_002936

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA