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IDH3A antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects IDH3A in WB. It exhibits reactivity toward Human, Mouse, Dog, Pig, Horse and Rabbit.
Catalog No. ABIN6742120

Quick Overview for IDH3A antibody (C-Term) (ABIN6742120)

Target

See all IDH3A Antibodies
IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Reactivity

  • 46
  • 33
  • 18
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Dog, Pig, Horse, Rabbit

Host

  • 41
  • 4
  • 1
Rabbit

Clonality

  • 43
  • 3
Polyclonal

Conjugate

  • 21
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This IDH3A antibody is un-conjugated

Application

  • 34
  • 13
  • 13
  • 13
  • 13
  • 10
  • 6
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    Human IDH3A

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Dog, Pig (100%) Rat, Bovine, Chicken (92%) Xenopus (84%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from C-Terminus of human IDH3A (P50213, NP_005521). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Elephant, Dog, Rabbit, Horse, Pig, Opossum, Platypus (100%), Marmoset, Rat, Bovine, Guinea pig, Turkey, Zebra finch, Chicken (92%), Xenopus (84%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

    Alternative Name

    IDH3A

    Background

    Name/Gene ID: IDH3A

    Synonyms: IDH3A, H-IDH alpha, NAD+-specific ICDH

    Gene ID

    3419

    NCBI Accession

    NP_005521

    UniProt

    P50213
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