MAP3K8 antibody (AA 35-84)

Catalog No. ABIN6742194

The Rabbit Polyclonal anti-MAP3K8 antibody has been validated for WB. It is suitable to detect MAP3K8 in samples from Human, Mouse, Cow and Horse.

Quick Overview for MAP3K8 antibody (AA 35-84) (ABIN6742194)

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Reactivity: Human, Mouse, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP3K8 Antibody

Binding Specificity: AA 35-84

Specificity: Human MAP3K8

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (86%) Mouse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human MAP3K8 (P41279, NP_005195). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Marmoset (93%), Galago, Bat, Elephant, Horse (86%), Mouse (84%), Panda (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP3K8

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: MAP3K8 / TPL2

Background: Name/Gene ID: MAP3K8
Subfamily: MAP3K
Family: Protein Kinase

Synonyms: MAP3K8, C-COT, Cancer Osaka thyroid oncogene, COT, Ewing sarcoma transformant, EST, MEKK8, Proto-oncogene c-Cot, TPL2, Tumor progression locus 2, Protein kinase cot, Tpl-2, ESTF

Gene ID: 1326

NCBI Accession: NP_005195

UniProt: P41279

Pathways: PI3K-Akt Signaling, TCR Signaling