KAP1 antibody (N-Term)

Catalog No. ABIN6742210

This anti-KAP1 antibody is a Rabbit Polyclonal antibody detecting KAP1 in WB. Suitable for Human, Cow and Monkey.

Quick Overview for KAP1 antibody (N-Term) (ABIN6742210)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KAP1 Antibody

Binding Specificity: N-Term

Specificity: Human TRIM28

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human TRIM28 (Q13263, NP_005753). Percent identity by BLAST analysis: Human, Chimpanzee, Marmoset, Bovine (100%), Gibbon, Mouse, Rat (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28 / KAP-1

Background: Name/Gene ID: TRIM28
Subfamily: TIF1
Family: Tripartite Motif

Synonyms: TRIM28, E3 SUMO-protein ligase TRIM28, KRIP-1, Nuclear corepressor KAP-1, KAP-1, KAP1, KRAB-interacting protein 1, TIF1B, RNF96, TIF1-beta, TIF1beta, Tripartite motif-containing 28, KRAB-associated protein 1, RING finger protein 96, TF1B, Tif1beta zinc finger protein, Tripartite motif containing 28

Gene ID: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus