PITX2 antibody (N-Term)

Catalog No. ABIN6742271

This Rabbit Polyclonal antibody specifically detects PITX2 in WB. It exhibits reactivity toward Human, Rat, Horse, Dog and Monkey.

Quick Overview for PITX2 antibody (N-Term) (ABIN6742271)

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Reactivity: Human, Rat, Horse, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PITX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PITX2 Antibody

Binding Specificity: N-Term

Specificity: Human PITX2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Horse (100%) Mouse (92%) Guinea pig (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PITX2 (Q99697, NP_700475). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Rat, Dog, Horse (100%), Mouse (92%), Guinea pig (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PITX2

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Alternative Name: PITX2 / RGS

Background: Name/Gene ID: PITX2

Synonyms: PITX2, All1-responsive gene 1, ARP1, Brx1, Homeobox protein PITX2, IGDS2, IRID2, Iridogoniodysgenesis type 2, IGDS, Pituitary homeobox 2, PTX2, ALL1-responsive protein ARP1, RGS, Solurshin, Otlx2, Paired-like homeodomain 2, RIEG, RIEG1, RS, IDG2, IHG2

Gene ID: 5308

NCBI Accession: NP_700475

UniProt: Q99697

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development