Src antibody (N-Term)

Catalog No. ABIN6742276

This Rabbit Polyclonal antibody specifically detects Src in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Monkey, Hamster and Rabbit.

Quick Overview for Src antibody (N-Term) (ABIN6742276)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Hamster, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Src Antibody

Binding Specificity: N-Term

Specificity: Human SRC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Dog, Pig, Guinea pig (92%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SRC (P12931, NP_938033). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster (100%), Gibbon, Galago, Elephant, Dog, Bat, Pig, Guinea pig (92%), Bovine (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: SRC

Background: Name/Gene ID: SRC
Subfamily: Src
Family: Protein Kinase

Synonyms: SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1

Gene ID: 6714

NCBI Accession: NP_938033

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling