Src antibody (AA 36-85)

Catalog No. ABIN6742277

The Rabbit Polyclonal anti-Src antibody has been validated for WB. It is suitable to detect Src in samples from Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Hamster, Rabbit, Guinea Pig and Bat.

Quick Overview for Src antibody (AA 36-85) (ABIN6742277)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Hamster, Rabbit, Guinea Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Src Antibody

Binding Specificity: AA 36-85

Specificity: Human SRC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human SRC (P12931, NP_005408). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Guinea pig (100%), Opossum, Turkey, Chicken (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: SRC

Background: Name/Gene ID: SRC
Subfamily: Src
Family: Protein Kinase

Synonyms: SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1

Gene ID: 6714

NCBI Accession: NP_005408

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling