KAP1 antibody (AA 216-265)

Catalog No. ABIN6742303

This Rabbit Polyclonal antibody specifically detects KAP1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Cow, Monkey, Horse, Guinea Pig, Bat, Chicken and Xenopus laevis.

Quick Overview for KAP1 antibody (AA 216-265) (ABIN6742303)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Monkey, Horse, Guinea Pig, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KAP1 Antibody

Binding Specificity: AA 216-265

Specificity: Human TRIM28

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog, Bovine, Rabbit, Horse (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human TRIM28 (Q13263, NP_005753). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig, Chicken, Platypus, Xenopus (100%), Zebrafish (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28 / KAP-1

Background: Name/Gene ID: TRIM28
Subfamily: TIF1
Family: Tripartite Motif

Synonyms: TRIM28, E3 SUMO-protein ligase TRIM28, KRIP-1, Nuclear corepressor KAP-1, KAP-1, KAP1, KRAB-interacting protein 1, TIF1B, RNF96, TIF1-beta, TIF1beta, Tripartite motif-containing 28, KRAB-associated protein 1, RING finger protein 96, TF1B, Tif1beta zinc finger protein, Tripartite motif containing 28

Gene ID: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus