MED27 antibody (AA 180-229)

Catalog No. ABIN6742404

This anti-MED27 antibody is a Rabbit Polyclonal antibody detecting MED27 in WB. Suitable for Human, Cow, Dog, Guinea Pig, Horse, Pig, Chicken, Hamster and Xenopus laevis.

Quick Overview for MED27 antibody (AA 180-229) (ABIN6742404)

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Pig, Chicken, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED27 Antibody

Binding Specificity: AA 180-229

Specificity: Human MED27 / CRSP8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Pig, Chicken (100%) Mouse, Rat (92%) Dog (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human MED27 (Q6P2C8, NP_004260). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Hamster, Elephant, Bovine, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%), Marmoset, Mouse, Rat, Platypus (92%), Dog, Rabbit (84%), Stickleback (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: MED27

Background: Name/Gene ID: MED27

Synonyms: MED27, CRAP34, CRSP complex subunit 8, Mediator complex subunit 27, TRAP37, p37 TRAP/SMCC/PC2 subunit, CRSP34, CRSP8

Gene ID: 9442

NCBI Accession: NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha