MED27 antibody (AA 180-229)

Catalog No. ABIN6742404

The Rabbit Polyclonal anti-MED27 antibody (ABIN6742404) specifically detects MED27 in WB. The antibody is reactive with Human, Cow, Dog, Guinea Pig, Horse, Pig, Chicken, Hamster and Xenopus laevis samples.

Quick Overview for MED27 antibody (AA 180-229) (ABIN6742404)

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Pig, Chicken, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED27 Antibody

Binding Specificity: AA 180-229

Specificity: Human MED27 / CRSP8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Pig, Chicken (100%) Mouse, Rat (92%) Dog (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human MED27 (Q6P2C8, NP_004260). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Hamster, Elephant, Bovine, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%), Marmoset, Mouse, Rat, Platypus (92%), Dog, Rabbit (84%), Stickleback (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: MED27

Background: Name/Gene ID: MED27

Synonyms: MED27, CRAP34, CRSP complex subunit 8, Mediator complex subunit 27, TRAP37, p37 TRAP/SMCC/PC2 subunit, CRSP34, CRSP8

Gene ID: 9442

NCBI Accession: NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha