Connexin 43/GJA1 antibody (C-Term)

Catalog No. ABIN6742701

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody has been validated for WB. It is suitable to detect Connexin 43/GJA1 in samples from Human, Mouse, Dog, Monkey, Rabbit, Guinea Pig, Pig and Bat.

Quick Overview for Connexin 43/GJA1 antibody (C-Term) (ABIN6742701)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Mouse, Dog, Monkey, Rabbit, Guinea Pig, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: C-Term

Specificity: Human GJA1 / Connexin 43

Predicted Reactivity: Percent identity by BLAST analysis: Human, Pig, Guinea pig (100%) Mouse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GJA1 (P17302, NP_000156). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Panda, Dog, Bat, Rabbit, Pig, Opossum, Guinea pig (100%), Rat, Hamster, Elephant, Bovine, Horse (92%), Mouse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1 / CX43 / Connexin 43

Background: Name/Gene ID: GJA1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA1, Connexin-43, DFNB38, Connexin 43, Gap junction alpha-1 protein, GJAL, HSS, Gap junction protein alpha 1, ODDD, ODD, AVSD3, CX43, HLHS1, ODOD, SDTY3

Gene ID: 2697

NCBI Accession: NP_000156

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization