MITF antibody (C-Term)

Catalog No. ABIN6742737

The Rabbit Polyclonal anti-MITF antibody has been validated for WB. It is suitable to detect MITF in samples from Human, Mouse, Dog, Rat, Rabbit, Horse, Guinea Pig, Monkey, Cow, Zebrafish (Danio rerio), Goat, Bat, Chicken, Pig and Hamster.

Quick Overview for MITF antibody (C-Term) (ABIN6742737)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse, Dog, Rat, Rabbit, Horse, Guinea Pig, Monkey, Cow, Zebrafish (Danio rerio), Goat, Bat, Chicken, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MITF Antibody

Binding Specificity: C-Term

Specificity: Human MITF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MITF (O75030-9, NP_000239). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Zebrafish (100%), Opossum, Lizard, Salmon (92%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: Name/Gene ID: MITF

Synonyms: MITF, BHLHe32, CMM8, MI, Waardenburg syndrome, type 2A, WS2, WS2A

Gene ID: 4286

NCBI Accession: NP_000239

UniProt: O75030

Pathways: Chromatin Binding