SIM2 antibody (N-Term)

Catalog No. ABIN6742777

This Rabbit Polyclonal antibody specifically detects SIM2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Chicken and Xenopus laevis.

Quick Overview for SIM2 antibody (N-Term) (ABIN6742777)

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIM2 Antibody

Binding Specificity: N-Term

Specificity: Human SIM2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SIM2 (Q14190, NP_005060). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Opossum, Guinea pig, Zebra finch, Chicken, Lizard, Xenopus, Zebrafish (100%), Orangutan, Tamarin, Hamster, Panda, Bovine, Bat, Pig, Platypus, Stickleback, Drosophila (92%), Beetle (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIM2

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Alternative Name: SIM2

Background: Name/Gene ID: SIM2

Synonyms: SIM2, BHLHe15, HMC29C01, HMC13F06, Transcription factor SIM2, Single-minded homolog 2, SIM

Gene ID: 6493

NCBI Accession: NP_005060

UniProt: Q14190