FECH antibody (AA 252-301)

Catalog No. ABIN6743073

This Rabbit Polyclonal antibody specifically detects FECH in WB. It exhibits reactivity toward Human and Monkey.

Quick Overview for FECH antibody (AA 252-301) (ABIN6743073)

Target: FECH (Ferrochelatase (FECH))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FECH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FECH Antibody

Binding Specificity: AA 252-301

Specificity: Human FECH

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Rabbit, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human FECH (P22830, NP_000131). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FECH

Target: FECH (Ferrochelatase (FECH))

Alternative Name: FECH / Ferrochelatase

Background: Name/Gene ID: FECH

Synonyms: FECH, FCE, Heme synthase, EPP, Heme synthetase, Protoheme ferro-lyase, Protoporphyria, Ferrochelatase, Ferrochelatase, mitochondrial

Gene ID: 2235

NCBI Accession: NP_000131

UniProt: P22830

Pathways: Transition Metal Ion Homeostasis