UBE2E1 antibody (AA 71-120)

Catalog No. ABIN6743145

The Rabbit Polyclonal anti-UBE2E1 antibody (ABIN6743145) specifically detects UBE2E1 in WB. The antibody is reactive with Human, Mouse, Rabbit, Dog, Guinea Pig, Rat, Horse, Drosophila melanogaster, Monkey, Bat, Chicken, Hamster, Pig and Xenopus laevis samples.

Quick Overview for UBE2E1 antibody (AA 71-120) (ABIN6743145)

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Reactivity: Human, Mouse, Rabbit, Dog, Guinea Pig, Rat, Horse, Drosophila melanogaster, Monkey, Bat, Chicken, Hamster, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2E1 Antibody

Binding Specificity: AA 71-120

Specificity: Human UBE2E1 / UBCH6

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Chicken, Xenopus (100%) Bovine, Pig, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human UBE2E1 (P51965, NP_003332). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Xenopus, Pike, Sea squirt, Drosophila, Beetle (100%), Bovine, Turkey, Trout, Salmon, Smelt, Stickleback, Zebrafish (92%), Orangutan, Frog, Catfish, Rice, Grape (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2E1

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Alternative Name: UBE2E1 / UBCH6

Background: Name/Gene ID: UBE2E1

Synonyms: UBE2E1, UBCH6, Ubiquitin-protein ligase E1, Ubiquitin carrier protein E1

Gene ID: 7324

NCBI Accession: NP_003332

UniProt: P51965

Pathways: Ubiquitin Proteasome Pathway