NEDD4-2 antibody (AA 431-480)

Catalog No. ABIN6743164

The Rabbit Polyclonal anti-NEDD4-2 antibody has been validated for WB. It is suitable to detect NEDD4-2 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Bat, Chicken, Monkey and Xenopus laevis.

Quick Overview for NEDD4-2 antibody (AA 431-480) (ABIN6743164)

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Bat, Chicken, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NEDD4-2 Antibody

Binding Specificity: AA 431-480

Specificity: Human NEDD4L

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa431-480 of human NEDD4L (Q96PU5-5, NP_056092). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Xenopus (100%), Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: NEDD4L / NEDD4-2

Background: Name/Gene ID: NEDD4L

Synonyms: NEDD4L, HNEDD4-2, KIAA0439, NEDD4-2, RSP5, NEDD4.2, NEDL3, Ubiquitin-protein ligase Rsp5

Gene ID: 23327

NCBI Accession: NP_056092

UniProt: Q96PU5

Pathways: Negative Regulation of Transporter Activity