ITCH antibody (AA 143-192)

Catalog No. ABIN6743170

The Rabbit Polyclonal anti-ITCH antibody has been validated for WB. It is suitable to detect ITCH in samples from Human, Cow, Horse and Monkey.

Quick Overview for ITCH antibody (AA 143-192) (ABIN6743170)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ITCH Antibody

Binding Specificity: AA 143-192

Specificity: Human ITCH

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Rabbit (92%) Bovine, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human ITCH (NP_113671). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Elephant, Dog, Rabbit, Guinea pig (92%), Galago, Bovine, Bat, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background: Name/Gene ID: ITCH

Synonyms: ITCH, AIF4, AIP4, DJ468O1.1, NAPP1, NFE2-associated polypeptide 1

Gene ID: 83737

NCBI Accession: NP_113671

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events