POLK antibody (AA 35-84)

Catalog No. ABIN6743491

This Rabbit Polyclonal antibody specifically detects POLK in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Pig.

Quick Overview for POLK antibody (AA 35-84) (ABIN6743491)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLK Antibody

Binding Specificity: AA 35-84

Specificity: Human POLK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig (100%) Bovine, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human POLK (Q9UBT6, NP_057302). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Horse, Pig (100%), Monkey, Bat (92%), Galago, Bovine, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: POLK

Background: Name/Gene ID: POLK

Synonyms: POLK, DINP, DNA polymerase kappa, DINB1, DINB protein

Gene ID: 51426

NCBI Accession: NP_057302

UniProt: Q9UBT6

Pathways: DNA Damage Repair