TCF7L2 antibody (AA 401-550) (HRP)

Catalog No. ABIN674358

The HRP-conjugated Rabbit Polyclonal anti-TCF7L2 antibody (ABIN674358) specifically detects TCF7L2 in ELISA, IHC (p) and IHC (fro). The antibody is reactive with Rat samples.

Quick Overview for TCF7L2 antibody (AA 401-550) (HRP) (ABIN674358)

Target: TCF7L2 (Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF7L2 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TCF7L2 Antibody (HRP)

Binding Specificity: AA 401-550

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TCF7L2

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TCF7L2

Target: TCF7L2 (Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2))

Alternative Name: TCF7L2

Background:

Synonyms: TCF4, TCF-4, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, hTCF-4, TCF7L2

Background: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.

Gene ID: 6934

UniProt: Q9NQB0

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus