MLH1 antibody (AA 35-84)

Catalog No. ABIN6743966

This anti-MLH1 antibody is a Rabbit Polyclonal antibody detecting MLH1 in WB. Suitable for Human, Mouse, Rat, Monkey, Hamster, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Pig, Xenopus laevis and Bat.

Quick Overview for MLH1 antibody (AA 35-84) (ABIN6743966)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human, Mouse, Rat, Monkey, Hamster, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Pig, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MLH1 Antibody

Binding Specificity: AA 35-84

Specificity: Human MLH1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Xenopus (100%) Bovine (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human MLH1 (P40692, NP_000240). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Guinea pig, Xenopus (100%), Bovine, Lizard, Drosophila (92%), Opossum, Turkey, Chicken, Platypus, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background: Name/Gene ID: MLH1

Synonyms: MLH1, COCA2, FCC2, HMLH1, HNPCC, HNPCC2, MutL protein homolog 1

Gene ID: 4292

NCBI Accession: NP_000240

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response