Cytokeratin 1 antibody (C-Term)

Catalog No. ABIN6744431

This anti-Cytokeratin 1 antibody is a Rabbit Polyclonal antibody detecting Cytokeratin 1 in WB, IHC and IHC (p). Suitable for Human and Monkey.

Quick Overview for Cytokeratin 1 antibody (C-Term) (ABIN6744431)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human KRT1 / Cytokeratin 1

Predicted Reactivity: Percent identity by BLAST analysis: Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human KRT1 (P04264, NP_006112). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset (100%), Rabbit (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: KRT1 / CK1 / Cytokeratin 1

Background: Name/Gene ID: KRT1
Family: Keratin

Synonyms: KRT1, 67 kDa cytokeratin, CK-1, CK1, Cytokeratin 1, Cytokeratin-1, Epidermolytic hyperkeratosis 1, EHK, EHK1, Hair alpha protein, EPPK, NEPPK, K1, Keratin-1, KRTA, Type-II keratin Kb1, Type II keratin, Keratin 1, KRT1A

Gene ID: 3848

NCBI Accession: NP_006112

UniProt: P04264