CBP antibody (AA 144-193)

Catalog No. ABIN6744446

The Rabbit Polyclonal anti-CBP antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CBP in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Chicken, Guinea Pig and Horse.

Quick Overview for CBP antibody (AA 144-193) (ABIN6744446)

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Chicken, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CBP Antibody

Binding Specificity: AA 144-193

Brand: IHC-plus™

Specificity: Human CREBBP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human CREBBP (Q4LE28, NP_001073315). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Marmoset (93%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CBP

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Alternative Name: CREBBP / CBP

Background: Name/Gene ID: CREBBP

Synonyms: CREBBP, CREB binding protein, CREB-binding protein, KAT3A, RSTS, Rubinstein-Taybi syndrome, RTS, CBP

Gene ID: 1387

NCBI Accession: NP_001073315

UniProt: Q92793

Pathways: TCR Signaling, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha