ACAA1 antibody (AA 36-85)

Catalog No. ABIN6744451

The Rabbit Polyclonal anti-ACAA1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ACAA1 in samples from Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Monkey.

Quick Overview for ACAA1 antibody (AA 36-85) (ABIN6744451)

Target: ACAA1 (Acetyl-CoA Acyltransferase 1 (ACAA1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACAA1 Antibody

Binding Specificity: AA 36-85

Brand: IHC-plus™

Specificity: Human ACAA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Rabbit, Horse, Guinea pig (100%) Dog, Bovine, Chicken (92%) Mouse, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human ACAA1 (P09110, NP_001598). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Rat, Panda, Rabbit, Horse, Guinea pig (100%), Gibbon, Elephant, Dog, Bovine, Opossum, Zebra finch, Chicken (92%), Lizard, Xenopus, Stickleback (90%), Mouse, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ACAA1

Target: ACAA1 (Acetyl-CoA Acyltransferase 1 (ACAA1))

Alternative Name: ACAA1

Background: Name/Gene ID: ACAA1

Synonyms: ACAA1, Acetyl-CoA acyltransferase 1, ACAA, Acetyl-CoA acyltransferase, Beta-ketothiolase, THIO, PTHIO

Gene ID: 30

NCBI Accession: NP_001598

UniProt: P09110

Pathways: Monocarboxylic Acid Catabolic Process