LIMD1 antibody (N-Term)

Catalog No. ABIN6744453

Product Details anti-LIMD1 Antibody

Target: LIMD1 (LIM Domains Containing 1 (LIMD1))

Binding Specificity: N-Term

Reactivity: Mouse, Human, Cow, Guinea Pig, Rat, Pig, Rabbit, Zebrafish (Danio rerio), Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human LIMD1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Dog (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIMD1 (Q9UGP4). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Bovine, Pig, Guinea pig, Platypus, Zebrafish (100%), Elephant, Panda, Dog, Bat, Opossum (92%), Rabbit, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LIMD1

Target: LIMD1 (LIM Domains Containing 1 (LIMD1))

Alternative Name: LIMD1 (LIMD1 Products)

Synonyms: AW822033 antibody, D9Ertd192e antibody, xlimd1 antibody, LIM domains containing 1 antibody, LIM domains containing 1 L homeolog antibody, LIMD1 antibody, Limd1 antibody, limd1 antibody, limd1.L antibody

Background: Name/Gene ID: LIMD1

Synonyms: LIMD1, LIM domains containing 1

Gene ID: 8994

UniProt: Q9UGP4

Pathways: Ribonucleoprotein Complex Subunit Organization