EHD4 antibody (AA 396-445)

Catalog No. ABIN6744457

This anti-EHD4 antibody is a Rabbit Polyclonal antibody detecting EHD4 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Monkey and Bat.

Quick Overview for EHD4 antibody (AA 396-445) (ABIN6744457)

Target: EHD4 (EH-Domain Containing 4 (EHD4))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EHD4 Antibody

Binding Specificity: AA 396-445

Brand: IHC-plus™

Specificity: Human EHD4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human EHD4 (Q9H223, NP_644670). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Platypus, Lizard (100%), Elephant, Turkey, Zebra finch (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EHD4

Target: EHD4 (EH-Domain Containing 4 (EHD4))

Alternative Name: EHD4

Background: Name/Gene ID: EHD4

Synonyms: EHD4, EH domain-containing protein 4, EH-domain containing 4, EH domain containing 4, HCA10, HCA11, PAST4, Ortholog of rat pincher, PAST homolog 4

Gene ID: 30844

NCBI Accession: NP_644670

UniProt: Q9H223